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华东理工大学魏东芝个人简介（华东理工大学生物工程学院导师魏东芝）

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魏东芝

生物化工专业：博士生导师/硕士生导师

生物化学与分子生物学专业：博士生导师/硕士生导师

生物工程领域：工程硕士导师

国家二级教授，鲁华生物技术研究所所长，生物工程学院学术委员会主任。

全国优秀科技工作者，国家重点学科-生物化工学科带头人，上海市重点学科-生物工程学科带头人，教育部跨世纪优秀人才、上海市科技领军人才，上海市优秀学科带头人,中科院“百人计划”入选者。中国微生物学会酶工程专业委员会副主任，教育部生物技术与生物工程专业教指委副主任，上海生物工程学会副理事长，中国生物工程学会理事，中国生物化学与分子生物学学会理事。享受国务院特殊津贴和上海市特殊津贴，中国科协“九大”代表。

2002-2016年连续3次主持国家“科技重大专项”（包括新药创制专项），负责完成国家863、973课题、自然科学基金、国家科技攻关、上海市重点项目等20余项重大重点项目。发表SCI收录论文200余篇（包括AngewandteChemie、MetabolicEngineering、GreenChemistry、AppliedandEnvironmentalMicorbiology、ChemicalCommunications等国际顶级学术期刊），他引4500多次，发明专利60余项。获得国家新药临床证书3项，实现产业化项目20余项。已培养研究生300余人。

获2015年国家技术发明二等奖，2013年第十五届中国国际工业博览会铜奖，2013年上海市技术发明一等奖，2012年和2010年上海市科技进步一等奖。

研究领域：

1.生物催化工程：新酶/生物催化剂的发现、改造及其功能的揭示，酶的结构计算及其重新设计，细胞生物催化剂的重构。

2.生物制药工程：包括蛋白/多肽药物高效表达与规模化制备平台技术；生物催化技术在生物制药领域的应用。

近期代表性论文

1GuoxiuZhang,PeiLiu,LeiZhang,WeiWei,XuedongWang,DongzhiWei,WeiWang,Bioprospectingmetagenomicsofamicrobialcommunityoncottondegradation:Miningfornewglycosidehydrolases,JournalofBiotechnology,2016234:35-42

2HuanZhang,LuluShi,JinpingLin,MingSun,DongzhiWei,Effectiveimprovementoftheactivityofmembrane-boundalcoholdehydrogenasebyoverexpressionofadhSinGluconobacteroxydans,BiotechnologyLetters,2016,38,7:1131-1138.

3WenyuanGao,HaiyangFan,LifengChen,HualeiWang,DongzhiWei,Efficientkineticresolutionofsecondaryalcoholsusinganorganicsolvent-tolerantesteraseinnon-aqueousmedium,BiotechnologyLetters2016,38,7:1165-1171.

4LiangMin,LeiZhang,XinJiang,WeiWei,DongzhiWei,WeiWang,ANewStrategyfortheEnzymaticDegradationofSudanDyesinNon-alkalineConditions,WaterAirAndSoilPollution20162276

5YuanyuanYang,JinpingLin,DongzhiWei,HeterologousOverexpressionandBiochemicalCharacterizationofaNitroreductasefromGluconobacteroxydans621H,MolecularBiotechnology,2016586:428-440

6SenwenDeng,XiaoqiangMa,MingSun,DongzhiWei,ErzhengSu,EfficientenzymaticsynthesisofampicillinusingmutantPenicillinGacylasewithbio-basedsolventglycerol,CatalysisCommunications,201679:31-34

7YujieChen,DongYi,ShuiqinJiang,DongzhiWei,Identificationofnovelthermostabletaurine-pyruvatetransaminasefromGeobacillusthermodenitrificansforchiralaminesynthesis,APPLIEDMICROBIOLOGYANDBIOTECHNOLOGY20161007:3101-3111

8NanLiu,MengYang,XiangzhaoMao,BozhongMu,DongzhiWei,Molecularcloningandexpressionofanew-neoagarobiosehydrolasefromAgarivoransgilvusWH0801andenzymaticproductionof3,6-anhydro-l-galactose,BiotechnologyandAppliedBiochemistry,2016632:230-237

9HuihuiSun,WenyuanGao,HualeiWang,DongzhiWei,Expression,characterizationofanovelnitrilasePpL19fromPseudomonaspsychrotoleranswithS-selectivitytowardmandelonitrilepresentinactiveinclusionbodies,BiotechnologyLetters2016383:455-461

10KaiWu,LifengChen,HaiyangFan,ZhiqiangZhao,HualeiWang,DongzhiWei,Synthesisofenantiopureepoxidebyonepotchemoenzymaticapproachusingahighlyenantioselectivedehydrogenase,TetrahedronLetters2016578:899-904

11WenyuanGao,KaiWu,LifengChen,HaiyangFan,ZhiqiangZhao,BeiGao,HualeiWang,DongzhiWei,Anovelesterasefromamarinemudmetagenomiclibraryforbiocatalyticsynthesisofshort-chainflavoresters,MicrobialCellFactories201615:41

12SenwenDeng,XiaoqiangMa,ErzhengSu,DongzhiWei,EfficientcascadesynthesisofampicillinfrompenicillinGpotassiumsaltusingwildandmutantpenicillinGacylasefromAlcaligenesfaecalis,JournalofBiotechnology2016219:142-148

13LeiZhang,XihuaZhao,GuoxiuZhang,JiajiaZhang,XuedongWang,SupingZhang,WeiWang,DongzhiWei,Light-induciblegeneticengineeringandcontrolofnon-homologousend-joininginindustrialeukaryoticmicroorganisms:LML3.0andOFN1.0ScientificReports20166:20761

14ShanLi,YushuMa,DongzhiWei,IdentificationofaninteractionbetweenEIandahistidinekinase-responseregulatorhybridproteininGluconobacteroxydans,BiochemicalandBiophysicalResearchCommunications20164702:331-335

15JianDeng,ZhiqiangYao,KanglingChen,Y.AdamYuan,JinpingLin,DongzhiWei,Towardsthecomputationaldesignandengineeringofenzymeenantioselectivity:AcasestudybyacarbonylreductasefromGluconobacteroxydans,JournalofBiotechnology,2016217:31-40

16JinmaoZhang,XinyiTao,JianwenLiu,DongzhiWei,YuhongRen,Fe3+-inducedbioinspiredchitosanhydrogelsforthesustainedandcontrolledreleaseofdoxorubicin,RSCAdvances2016653:47940-47947

17BeiGao,YouzhiMao,LujiaZhang,LeiHe,DongzhiWei,Anovelsaccharifyingalpha-amylaseofAntarcticpsychrotolerantfungiGeomycespannorum:Genecloning,functionalexpression,andcharacterization,STARCH-STARKE2016681-2:20-28

18ShihuiShen,YushuMa,YuhongRen,DongzhiWei,ConstructionofanOscillatorGeneCircuitbyNegativeandPositiveFeedbacks,JournalofMicrobiologyandBiotechnology2016261:139-144

19LeiZhang,SupingZhang,XinJiang,Wei,Wei,WeiWang,DongzhiWei,Anovelhost-vectorsystemforheterologousproteinco-expressionandpurificationintheTrichodermareeseiindustrialstrainRUT-C30BiotechnologyLetters2016381:89-96

20JianZhang,AiyouSun,RuiXu,XinyiTao,YuguoDong,XinxinLv,DongzhiWei,Cell-penetratingandendoplasmicreticulum-locatingTAT-IL-24-KDELfusionproteininducestumorapoptosis,JournalofCellularPhysiology20162311:84-93

21JianDeng，ZhiqiangYao，KanglingChen，Y.AdamYuan，JinpingLina*，DongzhiWei(*)，Towardsthecomputationaldesignandengineeringofenzymeenantioselectivity:AcasestudybyacarbonylreductasefromGluconobacteroxydans，JournalofBiotechnology，2016，217：31-40。

22XinGao,ChengchengZhao,TingYu,ShengliYang,YuhongRen,DongzhiWei*,Constructionofareusablemulti-enzymesupramoleculardeviceviadisulfidebondlocking.ChemicalCommunications,51(2015):10131-10133.

23DongbingCui,LujiaZhang(*),ShuiqinJiang,ZhiqiangYao,BeiGao,JingpinLin,YAdamYuan,DongzhiWei(*).AcomputationalstrategyforalteringanenzymeinitscofactorpreferencetoNAD(H)and/orNADP(H),FEBSJOURNAL,2015,2339-2351

24Chen,R.,Deng,J.,Lin,J.,Yin,X.,Xie,T.,Yang,S.,&Wei,D.AssessingthestereoselectivityofcarbonylreductasestowardthereductionofOPBEanddockinganalysis[J].Biotechnologyandappliedbiochemistry,2015.

25WuK,WangH,SunH,WeiD.EfficientkineticresolutionofphenylglycidyletherbyanovelepoxidehydrolasefromTsukamurellapaurometabola[J].Appliedmicrobiologyandbiotechnology,2015,99(22):9511-9521.

26ZhuS,MaX,SuE,WeiD.Efficienthydrationof2-amino-2,3-dimethylbutyronitrileto2-amino-2,3-dimethylbutyramideinabiphasicsystemviaaneasilypreparedwhole-cellbiocatalyst[J].GreenChemistry,2015,17(7):3992-3999.

27HualeiWang;HaiyangFan;HuihuiSun;LiZhao;DongzhiWei.ProcessDevelopmentfortheProductionof(R)-(?)-MandelicAcidbyRecombinantEscherichiacoliCellsHarboringNitrilasefromBurkholderiacenocepaciaJ2315[J].OrganicProcessResearch&Development,2015,19(12):2012-2016.

28XingxingZhang;BaoqiZhang;JinpingLin;DongzhiWei.OxidationofethyleneglycoltoglycolaldehydeusingahighlyselectivealcoholdehydrogenasefromGluconobacteroxydans[J].JournalofMolecularCatalysisB:Enzymatic,2015,112:69-75.

29RongChen;XuLiu;JialeWang;JinpingLin;DongzhiWei.Cloning,expression,andcharacterizationofananti-PrelogstereospecificcarbonylreductasefromGluconobacteroxydansDSM2343[J].Enzymeandmicrobialtechnology,2015,70:18-27.

30TingYu;XinGao;YuhongRen;DongzhiWei.Assemblyofcellulaseswithsyntheticproteinscaffoldsinvitro[J].BioresourcesandBioprocessing,2015,2(1):1-7.

31ZhongweiGuo;KefengNi;DongzhiWei;YuhongRen.Fe3+-inducedoxidationandcoordinationcross-linkingincatechol–chitosanhydrogelsunderacidicpHconditions[J].RSCAdvances,2015,5(47):37377-37384.

32JianDeng;KanglingChen;ZhiqiangYao;JinpingLin;DongzhiWei.EfficientsynthesisofopticallyactivehalogenatedarylalcoholsathighsubstrateloadusingarecombinantcarbonylreductasefromGluconobacteroxydans[J].JournalofMolecularCatalysisB:Enzymatic,2015,118:1-7.

33XiaoqiangM;SenwenDenga;ErzhengSub;DongzhiWei.One-potenzymaticproductionofdeacetyl-7-aminocephalosporanicacidfromcephalosporinCviaimmobilizedcephalosporinCacylaseanddeacetylase[J].BiochemicalEngineeringJournal,2015,95:1-8.

34JianbingYang;KefengNi;DongzhiWei;YuhongRen.One-steppurificationandimmobilizationofhis-taggedproteinviaNi2+-functionalizedFe3O4@polydopaminemagneticnanoparticles[J].BiotechnologyandBioprocessEngineering,2015,20(5):901-907.

35DongshuangWang;KefengNi;YuhongRen;DongzhiWei.Site-directedmodificationofgeneticallyengineeredProteussp.lipaseK107variantswithapolyethyleneglycolderivative[J].JournalofMolecularCatalysisB:Enzymatic,2015,111:36-42.

36MinChen;XiaoLei;ChuzeChen;ShiyangZhang;JingliXie;DongzhiWei.Cloning,overexpression,andcharacterizationofahighlyactiveendoinulinasegenefromAspergillusfumigatusCl1forproductionofinulo-oligosaccharides[J].Appliedbiochemistryandbiotechnology,2015,175(2):1153-1167.

37YuguoDong;RuiXu;LihuaWang;JianZhang;ChaogangBai;AiyouSun;DongzhiWei.Acombinedfeedingstrategyforenhancingmycophenolicacidproductionbyfed-batchfermentationinPenicilliumbrevicompactum[J].ProcessBiochemistry,2015,50(3):336-340.

38YouzhiMao;YanchenYin;LujiaZhang;SitiAisyahAlias;BeiGao;DongzhiWei.DevelopmentofanovelAspergillusuracildeficientexpressionsystemanditsapplicationinexpressingacold-adaptedα-amylasegenefromAntarcticfungiGeomycespannorum[J].ProcessBiochemistry,2015,50(10):1581-1590.

39YingYang;LujiaZhang;MingrongGuo;JiaqiSun;ShingoMatsukawa;JingliXie;DongzhiWei.Novelα-l-ArabinofuranosidasefromCellulomonasfimiATCC484andItsSubstrate-SpecificityAnalysiswiththeAidofComputer[J].Journalofagriculturalandfoodchemistry,2015,63(14):3725-3733.

40ChaoChen;YumeiWang;ChunSu;XinqingZhao;MingLi;XiaoweiMeng;YingyuJin;Seung-HwanYang;YushuMa;DongzhiWei;Joo-WonSuh.AntifungalactivityofStreptomycesalbidoflavusL131againsttheleafmoldpathogenPassalorafulvainvolvesmembraneleakageandoxidativedamage[J].JournaloftheKoreanSocietyforAppliedBiologicalChemistry,2015,58(1):111-119.

41BoYin;JianDeng;LirongLim;Y.AdamYuan;DongzhiWei.Structuralinsightsintostereospecificreductionofα,β-unsaturatedcarbonylsubstratesbyoldyellowenzymefromGluconobacteroxydans[J].Bioscience,biotechnology,andbiochemistry,2015,79(3):410-421.

42Sun,Huihui;Wang,Hualei;Gao,Wenyuan;Chen,Lifeng;Wu,Kai;Wei,Dongzhi.DirectedevolutionofnitrilasePpL19fromPseudomonaspsychrotoleransL19andidentificationofenantiocomplementarymutantstowardmandelonitrile[J].Biochemicalandbiophysicalresearchcommunications,2015,468(4):820-825.

43WangH;GaoW;SunH;ChenL;ZhangL;WangX;WeiD.ProteinEngineeringofaNitrilasefromBurkholderiacenocepaciaJ2315forEfficientandEnantioselectiveProductionof(R)-o-ChloromandelicAcid[J].Appliedandenvironmentalmicrobiology,2015,81(24):8469-8477.

44XinGao,ShuaiYang,ChengchengZhao,YuhongRen*,Dong-ZhiWei*，Artificialmultienzymesupramoleculardevice:highlyorderedself-assemblyofoligomericenzymesinvitroandinvivo，AngewandteChemie，2014，126（51）：14251-14254.

45Yao,Kang，Xu,Li-Qin，Wang,Feng-Qing*，Wei,Dong-Zhi*，Characterizationandengineeringof3-ketosteroid-Delta(1)-dehydrogenaseand3-ketosteroid-9alpha-hydroxylaseinMycobacteriumneoaurumATCC25795toproduce9alpha-hydroxy-4-androstene-3,17-dionethroughthecatabolismofsterols，MetabolicEngineering，2014，24：181-191.

46Zhang,Lujia，Tang,Xiaomang，Cui,Dongbing，Yao,Zhiqiang，Gao,Bei，Jiang,Shuiqin，Yin,Bo，Yuan,Y.Adam，Wei,Dongzhi*，Amethodtorationallyincreaseproteinstabilitybasedonthecharge-chargeinteraction,withapplicationtolipaseLipK107，ProteinScience，2014，23（1）：110-116.

47Yin,Bo，Cui,Dongbing，Zhang,Lujia，Jiang,Shuiqin，Machida,Satoru，Yuan,Y.Adam*，Wei,Dongzhi*，StructuralinsightsintosubstrateandcoenzymepreferencebySDRfamilyproteinGox2253fromGluconobateroxydans，Proteins:Structure,Function,andGenetics，2014，82（11）：2925-2935.

48Liu,Xu，Wang,Chao，Zhang,Lujia，Yao,Zhiqiang，Cui,Dongbing，Wu,Liang，Lin,Jinping*，Yuan,Yu-RenAdam，Wei,Dongzhi*，Structuralandmutationalstudiesonanaldo-ketoreductaseAKR5C3fromGluconobacteroxydans，ProteinScience，2014，23（11）：1540-1549.

49Gao,Xin，Ni,Kefeng，Zhao,Chengcheng，Ren,Yuhong*，Wei,Dongzhi*，Enhancementoftheactivityofenzymeimmobilizedonpolydopamine-coatedironoxidenanoparticlesbyrationalorientationofformatedehydrogenase，JournalofBiotechnology，2014，188：36-41.

50YouPY,QiuJ,SuEZ,WeiDZ*,CaricapapayaLipaseCatalysedResolutionofβ-AminoEstersfortheHighlyEnantioselectiveSynthesisof(S)-Dapoxetine,EUROPEANJOURNALOFORGANICCHEMISTRY,2013,3:557-565.

51YaoK,WangFQ,ZhangHC,WeiDZ*.Identi?cationandengineeringofcholesteroloxidasesinvolvedintheinitialstepofsterolscatabolisminMycobacteriumneoaurum.METABOLICENGINEERING,2013,15:75–87.

52NiK,ZhouX,ZhaoL,WangHL,RenYH*,WeiDZ*,MagneticCatechol-ChitosanwithBioinspiredAdhesiveSurface:PreparationandImmobilizationofv-Transaminase,PLoSONE,2012,7(7):e41101.

53ZhangC,FanK,ZhangW,ZhuR,ZhangL,WeiDZ*.Structure-basedcharacterizationofcanine-humanchimericuricasesanditsevolutionaryimplications.BIOCHIMIE.2012,94(6):1412-1420.

54LiuX,YuanZ,YuanY,LinJP,WeiDZ*.BiochemicalandstructuralanalysisofGox2181,anewmemberoftheSDRsuperfamilyfromGluconobacteroxydans.BIOCHEMICALANDBIOPHYSICALRESEARCHCOMMUNICATIONS.2011,415(2):410-415.

55WangJQ,TaoXY,ZhangYF,WeiDZ*,RenYH*etal.Reversionofmultidrugresistancebytumortargeteddeliveryofantisenseoligodeoxynucleotidesinhydroxypropyl-chitosannanoparticles.BIOMATERIALS31(15):4426-44332010

56WangFQ,ZhangCG,LiB,WeiDZ,TongWY*,NewMicrobiologicalTransformationsofSteroidsbyStreptomycesvirginiaeIBL-14,ENVIRONMENTALSCIENCE&TECHNOLOGY43(15):5967-59742009

57RenYH,WangY,ZhangYF,WeiDZ*etal,Overcomingmultidrugresistanceinhumancarcinomacellsbyanantisenseoligodeoxynucleotide-doxorubicinconjugateinvitroandinvivo,ConferenceInformation:7thInternationalSymposiumonPolymerTherapeutics,MAY26-28,2007CtrInvestPrincipeFelipe,Valencia,SPAIN,MOLECULARPHARMACEUTICS5(4):579-5872008

近期代表性专利

1.发明名称：一种微生物细胞转化法生产2-酮基-D-葡萄糖酸的方法，专利号：201310598049.7

2.发明名称：一种耐热葡萄糖淀粉酶及其编码基因与应用，专利号：ZL201310016408.3

3.发明名称：一种用于在里氏木霉细胞内表达外源蛋白的表达设备及其基因工程菌，专利号：201110177811.5

4.发明名称：3-甾酮-Δ1-脱氢酶和工程菌及应用，专利号：ZL201110024534.4

5.发明名称：胆固醇氧化酶基因和工程菌及应用，专利号：ZL201110024406.X

联系方式

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Tel:021-64252078

Fax:021-64250068

E-mail:dzhwei@ecust.edu.cn

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